![]() However, due to the presence of significant amounts of antibodies from the prophylactic preparations, the reactions after vaccine inoculation cannot be accurately evaluated using the ELISA method. ![]() In such cases, the immunological reaction is typically assessed after vaccine inoculation by measuring antibodies (proteins) using the ELISA method. However, antibody prophylaxis is not a complete substitute for vaccination, and it is recommended that regular vaccines be administered even in cases where prophylaxis is administered. In recent years, genetically engineered antibody preparations (tixagevimab/cilgavimab*2) have been widely administered as preventative drugs, primarily for cases with compromised immune systems where there is insufficient antibody production after inoculation with mRNA SARS-CoV-2 vaccines. By utilizing this analysis method to investigate immune responses following the administration of mRNA vaccines (mRNA formulations), it will be possible to widely evaluate the functionality of mRNA formulations.Detailed information can be derived, such as antibody epitopes expressed in immunological reactions or neutralization activity and the like, if there is additional information in respect of the antibodies in the databases used.This analysis is not the conventional analysis using protein level such as “the measurement of antibody titers using the ELISA method”, but analysis using mRNA level, and so evaluation is possible for samples with extremely elevated antibody titers after prophylactic administration of antibody preparations (proteins).While conducting the repertoire analysis, it was found that the immunological reactions could be evaluated by “sampling over time in the short time period wherein immunity is activated” and “utilizing the BCR gene sequence database for specific antigens”.Previously, B-cell receptor (BCR) repertoire analysis has provided a huge amount of gene sequence data of antibodies (B cell receptors), but it was difficult to use this data to evaluate the immunological reactions to specific antigens.These research results were published online in British Journal of Haematology on June 22, 2023. A new method to assess the status of immune responses to specific antigens in detail by analysis using the B cell receptor (BCR) repertoire(*1) has been developed by a research group, consisting of Assistant Professor Yohei Funakoshi, Associate Professor Kimikazu Yakushijin, Professor Hironobu Minami (Kobe University Graduate School of Medicine), Associate Professor Goh Ohji (Kobe University Hospital), and Researcher Takaji Matsutani (Repertoire Genesis Inc.).
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